Closed FluorCam FC-800-C

The closed FluorCam FC 800-C represents a highly innovative, robust, and easy-to-use system for combined multispectral and kinetic fl uorescence imaging. It consists of a CCD camera, four to five fixed LED panels (4 + 1 additional, which is not included in the standard configuration) and, optionally, a filter wheel equipped with up to 7 different emission filters. LED panels provide uniform irradiance over 90 × 90mm area, suitable for images of small plants (such as Arabidopsis thaliana), shed leaves, mosses, lichens, silver algae colonies or algae suspensions, etc. The system is very compact and allows easy adaptation to darkness of an investigated sample.
The closed FluorCam FC 800-C generates fluorescence signal images at any time during the experiment and displays them using a dummy color scale. Full kinetic analysis is available. In all applications, the camera enables images of fluorescence transients induced by actinic light or saturation flashes. The time and amplitude of the actinic irradiance are determined by user-defined protocols. The enclosed FluorCam FC 800-C also includes a high-performance PC and a comprehensive software package comprising total system control, data acquisition, and image processing. For a seasoned professional, the software offers a sophisticated programming language that can be used to design novel timing and measurement sequences.

Fluorescence parameters:
- Measured parameters: FO, FM, FV, FO ', FM', FV ', FT
- More than 50 calculated parameters: FV / FM, FV '/ FM', PhiPSII, NPQ, qN, qP, Rfd, PAR absorptivity coefficient and many others
Light sources:
455nm, 470nm, 505nm, 570nm, 605nm, 618nm, 630nm, 735nm, white and others

Super pulse intensity:
- 4,000 µmol. m-2. s-1 (in standard version)
- 6,000 µmol. m-2. s-1 (in light enhanced version)

Actinic light intensity:
Up to 2000 µmol. m-2. s-1 (in standard version)
Up to 3,000 µmol. m-2. s-1 (in light enhanced version)

Filter wheel:
7 positions

Light regime:
Static or harmonically modulated (sine waveform)

Custom defined protocols:
Variable tense, special language, and scripts.

CCD detector wavelength range:
400 - 1000 nm

CCD format:
- 720 x 560 pixels (TOMI-1 camera)
- 1360 x 1024 pixels (TOMI-2 camera)
- 1280 x 1024 pixels (TOMI-3 camera)

Pixel size:
- 8.60 µm x 8.30 µm (TOMI-1)
- 6.45 µm x 6.45 µm (TOMI-2)
- 6.60 µm x 6.60 µm (TOMI-3)

Bit resolution A / D:
- 12 bits (TOMI-1)
- 16 bits (TOMI-2)
- 12 bits (TOMI-3)

Interface connector:
Gigabit Ethernet

Outside dimension:
472mm (width) x 479mm (depth) x 513mm (height)

Weight:
Approx. 40 kilograms

Power input:
Approx. 1100 W

Electric:
90 - 240 V

Cameras:
High sensitivity TOMI-1 or high resolution TOMI-2 or high speed TOMI-3

QA-Reoxidation Module
High-end option allowing determination of QA-reoxidation kinetics. QA-reoxidation is recorded in a pump-and-probe manner, i.e. using repetitive flashes and recording with variable delay after each flash.
PAR-Absorptivity and NDVI Module
High-end option for measuring PAR absorptivity and NDVI reflectance index. It includes 7-position filter wheel, light panel 130 x 130 mm (installed round the camera and mounted with far-red LEDs 740 nm + deep-red LEDs 660 nm) and a glass filter. PAR-measurement is based on reflectance of plants in near infrared region and in the visible red range of spectrum. NDVI is used as vegetative index for predicting photosynthetic activity.
IMPORTANT NOTES: To be used only with FC 800-C/1010, FC 800-C/1010-GPF or FC 800-O/1010. This module cannot be used with the Multicolor Plant Module in FC 800-C/1010 or FC 800-C/1010-GPF.

Multicolor Plant Module
High-end option for measuring emission spectra in plants (according to Lichtenthaler and Bushmann). It includes 7-position filter wheel, UV LED light panel (installed round the camera and mounted with 4 UV LEDs 380 nm), four filters - BLUE (FF01-440/40), GREEN (FF02-520/28), RED (FF01-690/8), FAR-RED (FF01-747/33) - and a special software module.
IMPORTANT NOTES: To be used only in the FC 800-C/1010, FC 800-C/1010-GPF or FC 800-O/1010. This module cannot be used with the PAR-Absorptivity and NDVI Module in the FC 800-C/1010 or FC 800-C/1010-GPF.

Fast Fluorescence Induction (OJIP) Module
Electronic module for tracking fast fluorescence induction – OJIP curve. Includes a special electronic board and measuring protocol. The processes are recorded in multiple light exposures, each with a variable delay after the light is switched on.

Filter Wheel
7-position software-controlled filter wheel allows for imaging in different emission bands. Filters are easily interchangeable.

Additional LED Light Panels
Additional LED light panel is mounted at the top part of the FluorCam System. It is located under the camera; the objective of the camera is inserted in the central opening of this panel. The panel widens the possibility of employing more excitation colors for special applications. It is equipped with 4 high-power LEDs in one-channel version, or 8 high-power LEDs in two-channel version. Available colours are: far-red (740 nm), deep-red (660 nm), ultra-violet (365 or 385 nm), green (523 nm), amber (590 nm). Other options available upon request.
Ultraviolet Panel (365 or 385 nm): Recommended use: (1) multicolor fluorescence - UV excited blue F440, green F520, red F680, and infra-red F740 (must be accompanied with the filter wheel and high-resolution CCD); (2) excitation source for wtGFP, DAPI (filter wheel and high-resolution CCD required). Optical power 1,5 W.
Blue Panel (470 nm): Recommended use: (1) excitation source for EGFP (must be accompanied with the filter wheel and high-resolution CCD); (2) studies on stomata function.
Green Panel (530 nm): Recommended use: excitation source for YFP (must be accompanied with the filter wheel and high-resolution CCD).
Far-Red Panel (740 nm) : Maximum total output: 1,200 mW (4 x 300 mW). Recommended use: determination of Fo' and PAR absorbance (use of filter wheel required).
PAR - NDVI Panel (660 nm + 740 nm): This panel is part of the PAR-Absorptivity and NDVI Module.

Set of Grids for Sample Holders
Grids serve for firm and secure attachment of sample holders - Petri dishes or multi-well plates - to the desired spot on the FluorCam shelf or bottom. They are sold as a set of six different holders: for 90 mm, 100 mm, 120 mm, or 140 mm Petri dishes; 4 x 60 mm Petri dish; and for 96-well plate.

Additional Protective Glasses
Radiation safety glasses protecting against excessive LED radiation – equipped with side cover and protective filters. One pair of glasses is provided with the device free of charge.

Advanced Multiple Function for FluorCam Software
Software upgrade enabling multiple scripting functions for automatic initiation and measurement of scheduled FluorCam protocols and for automatic data storage. Advanced multiple function feature allows to define time of protocol initiation with user-defined protocol, apply user defined mask for feature segmentation, automatically subtract the background based on the mask definition and analyze the measured data. The are stored as TAR files with time and date description. Useful for circadian cycle study.

Comprehensive Module for Automatic and Programmable Image Acquisition and Data Management *
Comprehensive software and hardware package for data acquisition and image parameters extraction and analysis. Software part offers user friendly graphical interface, where complex protocols can be easily designed including dark/light adaptation schemes and executed based on user defined actions. All acquired data are stored in an SQL database, processed and available for inspection and further analysis in range of seconds after recording. System allows rapid and easy data browsing, grouping, analysis, user-defined data reprocessing and export. Hardware part includes PC with RAID5 data array, router, monitor, keyboard and mouse.

Barcode Reader with Supporting Software
Barcode reader with FluorCam supporting software included. Used for fully automatic bar code search.